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KMID : 0380420160400010012
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Journal of Prventive Veterinary Medicine
2016 Volume.40 No. 1 p.12 ~ p.21
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Canine mesenchymal stem cells immunomodulate atopic dermatitis through the induction of regulatory T cells in an ex vivo experimental study
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Byeon Jeong-Su
Lee Jienny
Kim Do-Hyung
Lee Gyeong-Been
Kim Hee-Ryang
Gu Na-Yeon
Cho In-Soo
Cha Sang-Ho
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Abstract
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Mesenchymal stem cells (MSCs) are multipotent stem cells, which can be induced to differentiate into several cells. MSCs are also reported to possess immunomodulatory properties through secretion of inflammatory cytokines and generation of regulatory T (Treg) cells. Treg cells play an important role in allergic disorders, including atopic dermatitis. We examined the immunomodulatory effects of canine adipose tissue derived-MSCs (cAD-MSCs) in 3 groups: Group 1, untreated normal dog; Group 2, dogs with Dermatophagoides farinae ointment-induced atopic dermatitis; and Group 3, dogs with atopic dermatitis. Canine peripheral blood mononuclear cells (PBMCs) were collected from each group and co-cultured with cAD-MSCs. After co-culturing, PBMCs were separated and the expression of Treg cells was examined by flow cytometry. According to the results, the populations of Treg cells were increased in 3 ex vivo experimental groups, co-cultured with cAD-MSCs. These results would be important for the application of MSCs in clinical trials.
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KEYWORD
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Canine adipose tissue, mesenchymal stem cells, PBMCs, Treg cells, immunomodulatory effects
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